DNA MICROSATELLITE ANALYSIS FOR PARENTAGE CONTROL IN AUSTRIAN PIGS

Abstract

We present an efficient parentage control for pigs based on ten polymorphic microsatellite markers analyzed in a single PCR reaction. Assuming one known parent (“paternity control”), combined exclusion probabilities (CEPs) ranged from 99.18% (Landrace), 99.74% (Piétrain) to 99.76% (Large White) for the most important Austrian breeds. Assuming a known parent-pair (“parentage control”, e.g. a substituted offspring), the CEP of the 10-plex PCR increased to 99.97% (Landrace) and 99.99% (Piétrain and Large White). We developed an additional standby battery of 5 markers, which might be applied in those cases, where the CEP of the 10-plex PCR is not sufficient. Therefore an automated, cost and time reduced genotype analysis for pigs is available.

ACKNOWLEDGMENTS

The authors would like to thank Denis Milan, INRA, France, for the French PigMap reference DNAs disposal. This work was supported by a grant to the Institute for Animal Breeding and Genetics, Vienna University of Veterinary Medicine, from the Austrian Federal Ministry of Agriculture and Forestry (No. 1151), by the Association of Austrian Pig Producers and by the H. Wilhelm Schaumann Stiftung.