DETERMINATION OF RUTIN IN HYPERICUM PERFORATUM EXTRACT BY CAPILLARY ELECTROPHORESIS

Abstract

A capillary zone electrophoretic (CZE) method for the determination of rutin in an ethanolic extract of the aerial parts of Hypericum perforatum (Hypericaceae) is described. The analysis was performed using a fused-silica capillary and a background electrolyte consisted of 10% ethanol and 20 mM borate buffer at pH 8.0 applied at a potential of 28 kV, vacuum injection of 1 s and detecting at 200 nm. Rutin had a migration time of 10.1 ± 0.2 min. Reproducibility was 1.26% for 9.37 × 10⁻⁵ M standard rutin. Detection limit was 2.7 × 10⁻⁶ M (S/N = 3). A well-correlated calibration equation was obtained for the mentioned conditions. The amount of rutin in the total plant material was found to be 0.21% ± 0.02.

Keywords:

• Rutin
• Capillary electrophoresis
• Analysis
• Flavonol glycosides

Acknowledgments