THIN LAYER CHROMATOGRAPHIC STUDY ON INCLUSION COMPLEX FORMATION BETWEEN CYCLODEXTRINS AND SOME FLAVONOIDS

Abstract

The interaction between two flavonoids and six cyclodextrins (CDs) was studied by reversed phase charge transfer thin layer chromatography. The NH₃ · H₂O‒NH₄Cl buffer containing various CDs (pH 9.7, 20°C) and polyamide plates were selected, respectively, as mobile and stationary phases. CDs, except for α-CD and CM-β-CD, can form inclusion complexes with flavonoids by this method. Furthermore, the inclusion capacity of different CDs was compared and the relative strength of interaction was calculated. The marked influence of CDs on the hydrophobility of drugs suggests that this interaction may modify the biological properties of drugs.

Keywords:

• Cyclodextrin
• Reversed phase thin layer chromatography
• Flavonoids
• Inclusion complex

ACKNOWLEDGMENTS

This work was supported by the National Natural Science Foundation of China and the National Natural Science Foundation of Shanxi province of China.