CHEMILUMINESCENCE ASSAY FOR BACTERIUM HELICOBACTER PYLORI DNA

Abstract

A chemiluminescence dot blot hybridization assay for Helicobacter pylori (H. pylori) DNA was established. A 20-mer oligo-nucleotide probe labeled directly alkaline phosphatase (AP) and highly sensitive chemiluminescence substrate for AP were used to detect H. pylori DNA. Preliminary H. pylori DNA testing was carried out on gastric biopsy specimens of 24 cases from patients. The results of detection were compared with that of histological examination and rapid urease test, showing the chemiluminescence dot blot hybridization assay is specific and more sensitive in eliminating false-negative. This method has much shorter detection period and simpler detection protocol than reported probe hybridization assays. It may be used in the H. pylori infection diagnosis as an alternative method for contrast.

Keywords:

• Dot blot hybridization
• Chemiluminescence assay
• Helicobacter pylori bacterium DNA detection

ACKNOWLEDGMENTS

This project was supported by China Postdoctoral Science Foundation.