DNA MODIFIED CARBON PASTE ELECTRODE FOR THE DETECTION OF 6-MERCAPTOPURINE

Abstract

A new method for the preparation of a DNA modified carbon paste electrode has been developed and its electrochemical behavior has been investigated. It is found that 6-mercaptopurine(6-MP) can be accumulated onto this electrode surface. This electrode has been used for the determination of 6-MP by differential pulse voltammetry. The linear range is between 5.0 × 10^-6 and 8.0 × 10^-5mol/L, with a detection limit of 2.0 × 10^-6mol/L. The relative standard deviation is 3.6% for eight successive determinations of 2.0 × 10^-5mol/L 6-MP. The determination of 6-MP tablets was also carried out and satisfactory results have been obtained.

Keywords:

• DNA
• Chemically modified electrode
• 6-Mercaptopurine
• Voltammetry

ACKNOWLEDGMENTS

The authors greatly appreciate the supports from National Natural Science Foundation of China (29835110), Doctoral fund of the National Education Ministry and Jiangsu Natural Science Foundation of China.