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Analytical Letters Volume 34, 2001 - Issue 8
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Original Articles

β-CYCLODEXTRIN ENHANCED FLUORIMETRY FOR A DETERMINATION OF ASCORBIC ACID

Jinghe Yang Key Lab for Colloid and Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University, Jinnan, 250100, ChinaCorrespondence[email protected]
,
Changxia Sun Key Lab for Colloid and Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University, Jinnan, 250100, China
,
Xia Wu Key Lab for Colloid and Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University, Jinnan, 250100, China
&
Yuxia Diao Key Lab for Colloid and Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University, Jinnan, 250100, China
Pages 1331-1339 | Received 02 Oct 2000, Accepted 06 Mar 2001, Published online: 02 Feb 2007
 
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Abstract

A simple, sensitive and selected fluorimetry for determination of ascorbic acid (AA) is described. The method is based on the reaction of ascorbic acid in alkaline medium with 2,3-diaminonaphthalene without oxidant. The addition of βcyclodextrin (β-CD) greatly enhance the fluorescence of the system. Under optimum conditions, a linear relationship was obtained between the fluorescence intensity and the concentration of ascorbic acid in the range of 0.05–600 μg/ml, the detection limit is 8 ng/ml. In AA-DAN-β-CD system, the inclusion complex is formed. Its inclusion constant is 1.1 × 106L2/mol2.

ACKNOWLEDGMENT

This work was supported by Natural Science Foundations of China and Shandong Province and by visiting scholar Foundation of Key Lab. in University.

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