HYDRO-ORGANIC AND MICELLAR-ORGANIC REVERSED-PHASE LIQUID CHROMATOGRAPHIC PROCEDURES FOR THE EVALUATION OF SULPHONAMIDES IN PHARMACEUTICALS

Abstract

Two reversed-phase liquid chromatographic procedures were developed for the analysis of ten pharmaceutical formulations used in medicine and veterinary, which contained one of the following sulphonamides: sulphacetamide, sulphadiazine, sulphaguanidine, sulphamethazine, sulphamethizole, sulphamethoxazole, or sulphathiazole. In both chromatographic modes, the same C₁₈ column was utilized and the mobile phase contained a low amount of acetonitrile and citric buffer at pH 3.0. In one of the procedures, the surfactant sodium dodecyl sul phate was added to the mobile phase. The presence of micelles allowed a decrease in the amount of organic solvent from 11% to 6%, and facilitated sample dissolution. The surfactant also gave rise to changes in the elution order and selectivity of the drugs. The analyses of the formulations were usually in good agreement with the claimed levels, and the results showed good precision. The procedures were simple and fast, the chromatographic runs took less than 15 min and the samples were injected directly into the chromatograph after dilution and filtration.

Keywords:

• Sulphonamides
• Pharmaceuticals
• Reversed-phase liquid chromatography
• Acetonitrile-water
• Acetonitrile-sodium dodecyl sulphate

ACKNOWLEDGMENTS

This work was supported by the DGES Project PB97-1384 from Spain.