SIMULTANEOUS CHIRAL SEPARATION AND DETERMINATION OF THE OPTICAL PURITY OF NAPROXEN AND METHYL NAPROXEN BY CAPILLARY ELECTROPHORESIS WITH DUAL-CYCLODEXTRIN SYSTEM AS CHIRAL SELECTOR

Abstract

A method of simultaneous enantiomeric separation of weak acidic naproxen and neutral methyl naproxen using both neutral hydroxypropyl-β-cyclodextrin(HP-β-CD) and charged sulfated β-cyclodextrin(SO₃-β-CD) as chiral additives by capillary electrophoresis was described. Chiral recognition mechanism was studied by investigation of the effect of chiral selectors concentration, pH, and electrophoretic polarity mode on separation of enantiomers. In a real sample under optimum conditions the optical purity for both precursor and product was determined as 88.3% and 94.9%, respectively. The limit of detection, linearity, and reproducibility of the method were also investigated in this paper.

Keywords:

• Chiral separation
• Optical purity testing
• Naproxen
• Methyl naproxen
• Dual-cyclodextrin system

ACKNOWLEDGMENT

We thank Mr. Xu Yi of the State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics of Chinese Academy of Sciences, for providing the racemic, isomers of naproxen and methyl naproxen, and enzyme catalyzed real sample.