APPLICATION OF MANGANESE-TETRASULFONATOPHTHALOCYANINE AS A NEW MIMETIC PEROXIDASE IN THE DETERMINATION OF HYDROGEN PEROXIDE BY CHEMILUMINESCENCE REACTION WITH LUMINOL

Abstract

A new promising mimetic peroxidase, manganese-tetra-sulfonatophthalocyanine (MnTSPc) has been synthesized and applied to the determination of hydrogen peroxide based on its catalytic effect on the chemiluminescence reaction between luminol and hydrogen peroxide. Under optimum conditions, the calibration graph has a linear range of 4.0 × 10⁻⁸–2.0 × 10⁻⁵ mol/l hydrogen peroxide, the detection limit of the method is 6.8 × 10⁻⁹ mol/l. At a hydrogen peroxide concentration of 1.0 × 10⁻⁶ mol/l, the relative standard deviation is 2.4% (n = 10).

Keywords:

• Chemiluminescence
• Flow injection analysis (FIA)
• Mimetic enzyme
• Manganese-tetrasulfinatophthalocyanine
• Hydrogen peroxide

ACKNOWLEDGMENTS

This work was supported by the Education Commission Natural Science Foundation of Anhui Province (No. 2000JL091).