A SIMPLE METHOD FOR CHIRAL SEPARATION OF EPHEDRINES USING (R)-1-NAPHTHYLGLYCINE AND 3,5-DINITROBENZOIC ACID AS STATIONARY PHASE

Abstract

High performance liquid chromatography (HPLC) method using Pirkle-type chiral stationary phase (CSP) was developed for the separation of the enantiomers of ephedrine, norephedrine and pseudoephedrine, which were central nervous stimulants. The resolutions were made using normal-phase methodology with a mobile phase consisting of n-hexane, 1,2-dichloroethane and methanol. Conditions for the experiments were optimized, including the content of methanol, 1,2-dichloroethane and acetic acid in mobile phase, and the effect of temperature on separation was also investigated.

Keywords:

• Enantiomers separation
• Pirkle-type chiral stationary phase
• Ephedrine
• Norephedrine
• Pseudoephedrine

ACKNOWLEDGMENTS

The authors like to acknowledge the National Institute for the Control of Pharmaceutical and Biological Products (People's Republic of China) for providing the standards of ephedrines for this work.