APPLICATION OF LIPOSOMES TOGETHER WITH THIN LAYER POTENTIOMETRY FOR DETECTION OF ODORANT COMPOUNDS INTERACTION WITH LIPID LAYER

Abstract

Herein we present a study on the application of a thin-layer potentiometry for detection of an interaction of odorants with liposome lipid layer. Three different kinds of liposomes: neutral (phosphatidylcholine + cholesterol), negatively charged (with dicetylphosphate) and positively charged (with sterylamine), were applied for study of the influence of charge on the interaction with odorants. The relative marker ion tetrabutylammonium (TBA⁺) release from the interior of liposomes upon stimulation by odorants was used as a measure of their interaction with the lipid layer.

Keywords:

• Thin layer potentiometry
• Liposomes
• Odorant compounds

ACKNOWLEDGMENTS

The authors acknowledge the State Committee for Scientific Research (KBN) for the financial support of the present work within the Grant no: 5 PO6 G 02615. The authors thank Prof. Yoshio Umezawa (University of Tokyo, Japan) for providing the equipment for the thin-layer potentiometry and Dr. Dariusz Kikut-Ligaj (Academy of Economy Chemistry Department, Poznan, Poland) for calculations by CHEM-X program.