81
Views
3
CrossRef citations to date
0
Altmetric
Original Articles

TWO-DIMENSIONAL TLC METHOD FOR IDENTIFICATION AND QUANTITATIVE ANALYSIS OF SALBUTAMOL AND RELATED IMPURITIES IN PHARMACEUTICAL TABLET FORMULATION

&
Pages 2099-2110 | Received 16 Feb 2001, Accepted 15 Jun 2001, Published online: 02 Feb 2007
 

Abstract

A rapid and sensitive TLC method was developed and validated for the analysis of salbutamol and identification of its related impurities compounds. Spectro-densitometric scanning-integration was performed at an absorbance wave length of 254 nm. To justify the suitability of the proposed method, accuracy, precision and recovery studies were performed at three selected concentrations levels. The recovery data reveals that the RSD for intra-day and inter-day analysis were found to be 4.86 and 1.23%, respectively. A TLC plastic plate precoated with silica gel was used as the stationary phase. The solvent system was acetonitrile : methanol : ammonium hydroxide (10 : 85 : 5 v/v/v) gave a dense and compact spots of salbutamol and related impurities compounds namely: isopropyl salbutamol, desoxy salbutamol base, salbutamol ketone hydrochloride and 5-formyl saligenin salbutamol with a Rf values of 0.1, 0.26, 0.35, 0.54 and 0.75 respectively. The calibration plots exhibited good linear relationship (r = 0.9996) over a concentration range of 5–25 mg/ml. Statical analysis proved that the proposed method is accurate and reproducible. The method is stability indicating and being economical can be employed for the routine analysis of the bulk material of salbutamol and its pharmaceutical tablets formulations.

ACKNOWLEDGMENT

The authors wish to thank the administration of King Faisal Specialist Hospital and Research Centre for its continuous support of the Pharmaceutical Analysis Laboratory research programs.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 768.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.