ABSTRACT
An amperometric glucose biosensor based on a coimmobilized electron transfer mediator (osmium-complex) layer, an enzyme (glucose oxidase) layer and a permeable pre-oxidation layer on a glassy carbon electrode was devised. The composition of the pre-oxidation layer was optimized. The permeable cellulose acetate membrane employing lead oxide as an interference-removing agent provided favorable permeability and was insusceptible to interference of ascorbic acid and uric acid. The biosensor exhibited high selectivity and a fast response and allowed a high working potential of +0.55 V vs. SCE. The amperometric response to glucose shows a linear relation in the range from 0 to 10 mM with a correlation coefficient of 0.9967. The biosensor was applied to the determination of glucose in human serum.
ACKNOWLEDGMENT
The financial support of the National Natural Science Foundation of China is gratefully acknowledged (No. 29975017).