MULTIPLEX PCR FOR SIMULTANEOUS DETECTION OF HUMAN CARCINOMA-RELATED VIRUSES

ABSTRACT

Multiplex PCR is proposed for simultaneous detection of the conserved genes of six human carcinoma-related viruses, which include HBV, HCV, EBV, HCMV, HSV-1 and HIV-1. The final concentration of various primers, which is one of the key factors affecting simultaneous amplification, was adjusted by using uniform design. Four loci, five loci and six loci of the target genes were successfully co-amplified. The optimized primer combination was applied to amplify viral genomes from blood sample or tissue culture. The method is to be developed further for clinical diagnosis and blood quality control.

Keywords:

• Multiplex PCR
• Uniform design
• Optimization
• Human carcinoma virus

ACKNOWLEDGMENTS

We thank Dr. Bing Zhu for the discussion of uniform design and supports from both the Chinese Academy of Sciences and the Ministry of Science and Technology, China.