SPECTROPHOTOMETRIC AND STABILITY INDICATING HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF NORTRIPTYLINE HYDROCHLORIDE AND FLUPHENAZINE HYDROCHLORIDE

ABSTRACT

Spectrophotometric and high performance liquid chromatographic procedures are described for determination of nortriptyline hydrochloride and fluphenazine hydrochloride. The first procedure is based on application of first derivative of ratio spectra (¹DD) for quantitative determination of nortriptyline hydrochloride in presence of fluphenazine hydrochloride. Secondly, an accurate, sensitive and stability indicating method has been introduced for determination of nortriptyline hydrochloride and fluphenazine hydrochloride in both bulk powder and in dosage form. In the derivative ratio method, Beer's law is obeyed in the concentration ranges of 8–32 µg mL⁻¹ and 4–32 µg mL⁻¹ of nortriptyline hydrochloride at wavelengths 271.4 and 284.2 nm, respectively. In high performance liquid chromatographic method, linear relationship in the range of 0.6–3.6 µg mL⁻¹ and 1.2–4.2 µg mL⁻¹ for nortriptyline hydrochloride and fluphenazine hydrochloride, respectively, was obtained. The mobile phase used was 0.05 M ammonium acetate : methanol : acetonitrile (4 : 1 : 5 v/v/v), and detection was done spectrophotometrically at 254 nm. Results were statistically analyzed and compared with those obtained by applying the British Pharmacopoeia (2000) method.

Keywords:

• Nortriptyline hydrochloride
• First derivative of ratio spectra
• Spectrophotometry (¹DD)
• Stability indicating HPLC method
• Determination of nortriptyline hydrochloride and fluphenazine hydrochloride

Acknowledgments