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ABSTRACT
A rapid and efficient method for extraction and isolation of microcystin-LR from the cyanobacterium is described. The method involves supercritical fluid extraction (SFE) for the fast extraction and silica gel flash chromatography for the purification of the microcystin-LR. The microcystin-LR can be successfully extracted with aqueous methanol modified supercritical carbon dioxide fluid. The procedure results in a purity of up to 95% microcystin-LR with the following preparative HPLC purification steps. The advantage of the method developed here is that the sample handling steps are minimized, thus reducing possible losses of microcystin-LR and saving extraction and purification time.
ACKNOWLEDGMENT
This work was supported by a grant from Korea Research Foundation (KRF 2001-002-D00220).