DETERMINATION OF TOTAL PROTEIN CONTENT IN HUMAN SERUM SAMPLES WITH FAST RED VR BY RESONANCE LIGHT SCATTERING TECHNIQUE

ABSTRACT

In an acidic medium, the interaction of Fast red VR (FRV) with proteins such as bovine serum albumin (BSA), human serum albumin (HSA), pepsin (Pep), α-chymotrypsin (Chy), and lysozyme (Lys) was characterized by measuring enhanced resonance light-scattering (RLS) signals. It was found that the enhanced RLS intensities of FRV by proteins at 287.0 nm were in proportion to the concentrations of proteins above, and the limits of determination for BSA, HSA, Lys, γ-IgG were below 25 ng/mL. The present method was successfully applied to the determinations of proteins in synthetic samples, and the total content of proteins in human blood plasma samples, respectively.

Keywords:

• Resonance light scattering (RLS)
• Fast red VR (FRV)
• Proteins

ACKNOWLEDGMENTS

The authors thank the financial supports from the Excellent Young University Teachers Foundation directed under the Education Ministry of China (No: 2000-11) and the Municipal Science Foundation of chongqing.