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Original Articles

SPECTROFLUORIMETRIC DETERMINATION OF REDUCED GLUTATHIONE IN HUMAN BLOOD USING N-[ p-(2-BENZOTHIAZOYL)-PHENYL]MALEIMIDE

, , , &
Pages 2269-2278 | Received 19 Jun 2002, Accepted 05 Jul 2002, Published online: 02 Feb 2007
 

ABSTRACT

A new method of the fluorimetric determination of reduced glutathione (GSH) has been developed with N-[ p-(2-benzothiazoyl)-phenyl]maleimide (BTPM). The maximal fluorescence for the reagent and the adduct were both at excitation and emission wavelengths of 323.2 and 388.8 nm, respectively. BTPM reacted with GSH to give a highly fluorescent derivative at 50°C for 35 min in H3BO3 (KCl)–Na2CO3 buffer (pH = 7.50). The relative fluorescence intensity (RF) was linear in the range of the GSH concentration from 4.00 × 10−9 to 2.40 × 10−7 mol L−1. The detection limit (DL) was 3.20 × 10−10 mol L−1 (S/N = 3). The proposed method has been applied to the determination of GSH in human blood and satisfactory results were obtained.

ACKNOWLEDGMENTS

This work was supported by the National Natural Science Foundation of China (No. 29575206, Beijing China), the Natural Science Foundation of Hubei Province (Wuhan, China) and the Foundation of Wuhan University (Wuhan, China).

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