High Performance Centrifugal Partition Chromatographic Separation of Cu(II), Mn(II), Co(II), and Ni(II) Using Di-2-Methylnonylphosphoric Acid

Abstract

A novel and simple method has been developed for the mutual separation of such metal ions as Cu(II), Mn(II), Co(II), Ni(II) by high performance centrifugal partition chromatography (HPCPC) with di-2-methylnonylphosphoric acid/heptane as a stationary phase. The HPCPC system was operated with 2,136 partition channels, at a rotational speed of 800 rpm, and at a flow rate of 2.0 cm³/min. The four transition metal ions could be eluted separately by changing pH of the chloroacetic acid mobile phase at two steps. The elution curves were obtained by monitoring the absorbance of each metal complex post-labeled with 4-(2-pyridylazo)resorcinol.