Determination of Kinetic Constants and Enzyme Activity from a Biosensor Transient Signal

Abstract

The kinetic constants and activity of soluble and immobilized enzymes can be determined electrochemically with a biosensor by applying the dynamic integrated model, which includes the inertia of the registering device, for data management and calculations. This model of electrochemical diffusion-limited sensors allows the prediction of steady-state parameters for enzyme-catalyzed reactions from a biosensor transient response and reproducible calculation of real physico-chemical constants without any additional experimental data. This approach has been used for the estimation of activity parameters of soluble and immobilized on mica glucose oxidase and for the evaluation of immobilization efficiency.

Keywords:

• Biosensor
• Transient signal
• Actual kinetic and diffusion constants
• Enzyme
• Immobilization

Acknowledgments

This work was financially supported by the Estonian Science Foundation (grant no. 5289).