Simple, Rapid, and Sensitive Spectrofluorimetric Determination of Zaleplon in Micellar Medium

Abstract

The influence of micellar medium on the absorption, fluorescent excitation and emission spectra character of Zaleplon was studied. The nonionic surfactant of Triton X-100 showed a strong sensitizing effect for the fluorescence of Zaleplon in a pH 5.0 buffer. The possible enhancement mechanism was discussed. Based on the optimum conditions, a simple, rapid, sensitive and selective spectrofluorimetric method (λ_ex/λ_em = 345/455 nm) has been developed for the determination of Zaleplon. The linear range was 1.32 × 10⁻⁸–1.00 × 10⁻⁵  mol/L. The detection limit was 4.0 × 10⁻⁹ mol/L with an RSD of 0.06%. The proposed method was successfully applied to the determination of Zaleplon in tablets, serum and urine.

Keywords:

• Zaleplon
• Micelle enhanced
• Fluorimetry

Acknowledgment

This work was supported by the Importance Natural Science Foundation of Shandong Province in China (no. Z2000B03).