Recent Innovations and Analytical Applications of Phage Display Libraries

Abstract

Filamentous phages are used widely for research and analytical technology development. This is due to the unique biology of the f-phages including its single stranded DNA genome and ability to display proteins on the surface of the intact phage particle, which have advanced the fields of recombinant DNA technology and molecular diversity technologies, respectively. Herein we review recent trends in phage display technologies that allow the evolution of selected binders into optimized molecules. Using this technology large polypeptide libraries are presented on the surface of filamentous phages. The selection of peptides and antibodies from phage display libraries has proven significant for routine isolation of peptides and antibodies for diagnostic and therapeutic applications.

Keywords:

• Bacteriophages
• Phage display
• Diagnosis
• Therapy
• Bacteria