Voltammetric Behavior of Indinavir and Determination in Pharmaceutical Dosage Forms

ABSTRACT

The oxidative behavior of indinavir has been investigated by cyclic, linear sweep, differential pulse, and square‐wave voltammetry using a glassy carbon electrode in different buffer systems. Cyclic voltammetry was used to study the influence of pH on the peak current and peak potential. The solution conditions and instrumental parameters were optimized to obtain a good sensitivity. The Britton–Robinson buffer of pH 6.0 was selected as a suitable analytical medium in which indinavir exhibited a sensitive diffusion controlled oxidative peak at +892.0 mV (vs. Ag/AgCl). The oxidation process was shown to be irreversible. The peak current varied linearly with drug concentration in the range between 2.8 × 10⁻⁷ and 5.0 × 10⁻⁵ M. The proposed voltammetric techniques have been applied to the determination of the drug in pharmaceutical dosage forms with good recoveries. For comparative purposes a HPLC with a diode array and multiple wavelength UV/VIS detection determination also was developed.

Keywords:

• Indinavir
• Cyclic voltammetry
• Linear sweep voltammetry
• Differential pulse voltammetry
• Square‐wave voltammetry
• Pharmaceutical dosage form
• HPLC

Notes

^a Y = a + bC where C is concentration in M and Y in peak area and current units for and voltammetric methods, respectively.

^aTablet, 200.0 mg per tablet (Crixivan^® capsule).

^bEach value is the mean of ten experiments.