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Analytical Letters Volume 37, 2004 - Issue 1
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Original Articles

Analysis of Mixture of Catechins, Flavones, Flavanones, Flavonols, and Anthocyanidins by RP‐HPLC

Ryszard Baranowski Department of Analytical and General Chemistry, Silesian Technical University, 44–100 M. Strzody 7, Gliwice, Poland
,
Joanna Kabut Department of Analytical and General Chemistry, Silesian Technical University, 44–100 M. Strzody 7, Gliwice, Poland
&
Irena Baranowska Department of Analytical and General Chemistry, Silesian Technical University, 44–100 M. Strzody 7, Gliwice, Poland
Pages 157-165 | Received 01 Aug 2003, Accepted 01 Sep 2003, Published online: 16 Aug 2010
 
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ABSTRACT

A reversed‐phase high performance liquid chromatographic (RP‐HPLC) separation system with diode array detector (DAD) was used to determine and quantify 21 flavonoids as aglycones and glycosides from five major flavonoid types (anthocyanidins, flavonols, flavanones, flavones, and catechins). The flavonoids were determined in freeze‐dried samples of fruit and vegetables. Identification of particular flavonoids in real samples was carried out by standard addition method and by comparing absorption spectra.

ACKNOWLEDGMENT

This work was supported by the State Committee for Scientific Research (Poland) project No.4#T09A#08922.

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