An Analytical Application of Ofloxacin by Solid‐Substrate Room Temperature Phosphorescence

ABSTRACT

This article presents a new determination method for the analysis of ofloxacin (OFLX) by solid‐substrate room temperature phosphorescence (SS‐RTP) with filter paper as solid substrate and CdCl₂ as heavy‐atom salt. Various factors affecting RTP intensity of OFLX, such as heavy‐atom effect, pH, and drying time of the sample, were studied in detail. The linear dynamic range (LDR), limit of detection, and RSD were: 1.03 to 411 ng/spot, 0.123 ng/spot and 1.7% for OFLX, respectively. The method was applied to determine OFLX in urine successfully. The recoveries of OFLX in urine were from 94.60% to 105.4%. The pharmacokinetic of OFLX was studied in urine as well. Mean accumulative excretion rate was 77.69%.

Keywords:

• Ofloxacin
• Room temperature phosphorescence
• Filter paper
• Urinary pharmacokinetic

ACKNOWLEDGMENT

This work was supported by the National Natural Science Foundation of China (No. 20275022).

Notes

^a I _p is the RTP intensity of OFLX, I _b is the background intensity of filter paper.

^bPaper sources are Chinese‐made quantitative middle (M), fast (F), slow (S) speed, and qualitative filter papers.

^aThe concentration of AgNO₃ is 0.1 mol/L, other salts are all 1.0 mol/L.

^b f _HA = I _p/I ₀, I _p, net intensity with heavy atom; I ₀, net intensity without heavy atom.