The Solid Surface Room Temperature Phosphorescence of Three Purine Compounds and Analytical Application

Abstract

Solid surface room temperature phosphorescence (SS‐RTP) of 6‐mercaptopurine (6‐MP), azathiopurine (BAN), and 8‐azaguanine (8‐Azan) was investigated. Various factors affecting the SS‐RTP signal were discussed in detail. The corresponding analytical determination methods were established. The linear dynamic range (LDR) was 2.00 × 10⁻³ mmol/L–0.401 mmol/L for 6‐MP, 2.01 × 10⁻³ mmol/L–0.600 mmol/L for BAN, 1.09 × 10⁻³ mmol/L–0.218 mmol/L for 8‐Azan, respectively. The limit of detection (LOD) was 1.30 µmol/L for 6‐MP, 1.21 µmol/L for BAN, 7.35 × 10⁻² µmol/L for 8‐Azan, respectively. Average recoveries in urine sample were 97.1%–99.9% for 6‐MP, 95.4%–101.7% for BAN, 96.2%–101.3% for 8‐Azan. The recovery of 6‐MP added to pharmaceutical preparation was 99.6%–100.5%.

Keywords:

• 6‐Mercaptopurine
• Azathiopurine
• 8‐Azaguanine
• Solid surface room temperature phosphorescence

Acknowledgments

This work was supported by the National Science Foundation of China (No. 20275022) and Shanxi Province Foundation of China (No. 20011010).