Analysis of Cerivastatin in Human Plasma and Identification of Its Lactone Metabolite by Capillary Electrophoresis

Abstract

A simple, fast, inexpensive, and reproducible method was developed for the analysis of cerivastatin in human plasma and identification of its metabolite by capillary electrophoresis (CE). The back ground electrolyte (BGE) used was phosphate buffer (50 mM, pH 3.5) with 15 kV as the applied voltage. The detection was achieved by using UV mode at 254 nm and the detection limit of cerivastatin and its metabolite was approximately 5 µg mL⁻¹. The separation and resolution factors were 1.07 and 4.01, respectively. The recovery of cerivastatin from plasma using SPE was 80%.

Keywords:

• Cerivastatin
• Cerivastatin metabolite
• Capillary electrophoresis
• Solid phase extraction

Acknowledgment

The authors (I.A. and H.Y.A.‐E.) would like to thank the King Faisal Specialist Hospital and Research Center, Riyadh administration for their support for the Pharmaceutical Analysis Laboratory Research Program.