Abstract
A bioelectrode system consisting of polyaniline (PAn)‐doped glassy carbon electrode (GCE) and cytochrome P4502D6 (CYP2D6) enzyme solution was used for the amperometric study of in vitro fluoxetine biotransformation. The PAn film was potentiostatically grown at +700 mV vs. Ag/AgCl (20°C) on a 0.071 cm2 GCE and used for cyclic voltammetric (CV) measurements in phosphate buffer solution (0.1 M, pH 7.5, 0.1 M KCl) of the enzyme. The response of the CYP2D6 bioelectrode to fluoxetine was consistent with uncompetitive substrate inhibition kinetics. Apparent Michaelis–Menten constant K′ m of the CYP2D6 electrode was 3.7 µmol/L, which is within the intra‐hepatic fluoxetine concentration between 2 and 7 µmol/L. Thus PAn‐mediated electrochemistry can be used to observe the monooxygenation reaction of CYP2D6. K′ m is the apparent Michaelis–Menten constant and K′ i is the apparent substrate inhibition constant.
Acknowledgment
We acknowledge National Research Foundation, South Africa and the University of the West Indies for Research grants.