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Abstract
Acridine orange was electropolymerized on a glassy carbon electrode by using a potentiodynamic technique to construct a catalytic interface for the investigation of dobutamine (DBTM). The electrochemical behaviors of DBTM on the modified electrode were studied by determining the influences from solution acidity, the scan rate, the accumulation time, and the working temperature. Experiments showed that two oxidation peaks (P1 and P2) and one reduction peak (P3) appeared for DBTM on the poly(acridine orange) film (PAO) modified electrode. The peak current of P1 exhibits a linear relationship with the variation of DBTM concentration in a wide concentration range (5.0 × 10−8 to 1.0 × 10−4 M). Under the optimum conditions, the detection limit has been estimated as 2.0 × 10−9 M with 200 sec open circuit accumulation. This method has been used successfully for the detection of DBTM in injections due to its good reproducibility and simplicity.
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