Flow‐Injection Spectrophotometric Determination of Amoxcillin, Cephalexin, Ampicillin, and Cephradine in Pharmaceutical Formulations

Abstract

Amoxcillin, cephalexin, ampicillin, and cephradine were determined spectrophotometrically in the pure form and in the pharmaceutical formulations by using flow‐injection analysis (FIA). The method is based upon the hydrolysis of the drug under investigation in basic medium and then the reduction of the formed hydrolyzed product by the on‐line generated I₂ in acidic medium. The decrease in the intensity of the I₂ color was monitored at 460 nm and found to be proportional to the concentration of the compound under investigation. Variables such as acidity, reagent concentrations, flow rate of reagents, and other FI parameters were optimized to produce the most sensitive and reproducible results. The method was successfully applied to the analysis of pharmaceutical preparations.

Keywords:

• Amoxcillin
• Cephalexin
• Ampicillin
• Cephradine
• Spectrophotometric
• Flow‐injection analysis
• Pharmaceutical analysis

Acknowledgments

The financial support from Yarmouk University is gratefully acknowledged. The author would like to thank Al‐Hikma pharmaceuticals, Amman‐Jordan, for providing the standard and the excipients.