Spectrophotometric Determination of Peroxynitrite Using o‐Phenylenediamine as a Probe

Abstract

In the present study, a novel spectrophotometric method for the determination of peroxynitrite using, o‐phenylenediamine as a probe is described. The method is based on the oxidation of o‐phenylenediamine, a colorless substance, by peroxynitrite to give a colored product. The absorbance increase of the reaction system is linearly related to the concentration of peroxynitrite in the range of 4.4 × 10⁻⁷–8.0 × 10⁻⁶ mol l⁻¹ with a detection limit of 1.7 × 10⁻⁷ mol l⁻¹ (3σ). The optimal conditions for the detection of peroxynitrite were evaluated and the possible detection mechanism was also discussed in this paper.

Keywords:

• Peroxynitrite
• o‐Phenylenediamine
• Spectroscopy
• Probe

Acknowledgments

The authors gratefully acknowledge the support of this research by a grant (No. 39770200) from the National Natural Science Foundation of China.