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Analytical Letters Volume 37, 2004 - Issue 14
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Original Articles

Isolation of Structurally Similar Citrus Flavonoids by Flash Chromatography

Girija Raman Texas A&M University–Kingsville, Citrus Center, 312 N. International Blvd., Weslaco, Texas, 78596, USA; Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, Texas, USA
,
G. K. Jayaprakasha Texas A&M University–Kingsville, Citrus Center, 312 N. International Blvd., Weslaco, Texas, 78596, USA; Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, Texas, USA
,
Jennifer Brodbelt Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas, USA
,
Minhee Cho Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas, USA
&
Dr. Bhimanagouda S. Patil Texas A&M University–Kingsville, Citrus Center, 312 N. International Blvd., Weslaco, Texas, 78596, USA; Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, Texas, USACorrespondence[email protected]
Pages 3005-3016 | Published online: 22 Aug 2007
 
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Abstract

A rapid and efficient method for the extraction and isolation of naringin and narirutin from citrus molasses is described. Naringin and narirutin are structurally similar glucosides present in citrus molasses. The common problem in purification of plant extracts is the large number of constituents present that are similar in nature. A flash chromatographic technique has been developed for the separation of the main flavonoid glucosides present in citrus molasses. This method is a rapid, inexpensive technique and provides compounds with high purity that can be used for biological activity studies. The separation and yield of narirutin and naringin obtained by this method is reproducible.

Acknowledgements

We thank Dr. Farzad Deyhim (Texas A&M University–Kingsville) and Dr. Kil Sun Yoo (Texas A&M University) for critically reviewing the manuscript. Financial support was provided by the ATP grant #2001‐003658‐0359 and USDA grant #2001‐52102‐11257 and 2001‐34402‐10543.

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