Amperometric Detection of Glucose Involving Electropolymerized Tetraaminophthalocyanine and Ferrocene Films

Abstract

Electropolymerized films of metal‐free tetraaminophthalocyanine (TAPc) were used to immobilize the redox mediator ferrocene (Fc) into its polymeric matrix during the electropolymerization of the monomer TAPc. This entrapment produces a three‐dimensional network of Fc active sites used for the detection of glucose. Comparative studies on electrochemical polymerization and characterization of TAPc and TAPc/Fc show significant differences in their charge transfer properties. Cyclic voltammetric characterization of TAPc films show irreversible charge transfer behavior while TAPc/Fc films show Nernstian equilibrium charge transfer properties. The TAPc/Fc films were used in the detection of glucose after the immobilization of glucose oxidase unto the TAPc/Fc layer. Studies on the bilayered enzyme electrode under stirred hydrodynamic conditions in aqueous glucose solutions produced a limiting current within 10–15 s.

The authors wish to acknowledge funding from the Michigan Space Grant (MSG 24124‐7080) and the Beckman Scholars Program.

Keywords:

• Chemically modified electrodes
• electropolymerization
• glucose
• ferrocene

Notes

The authors wish to acknowledge funding from the Michigan Space Grant (MSG 24124‐7080) and the Beckman Scholars Program.