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Abstract
An amperometric ferrocene‐mediated glucose biosensor was developed using the cross‐linking method with glutaraldehyde and bovine serum albumin (BSA) on carbon film electrodes fabricated by pyrolytic deposition of carbon. Ferrocene was mixed with the enzyme mixture or incorporated afterwards by potential cycling; the addition of Nafion to the mixture or coating the biosensor assembly with Nafion was also tested. The optimized operating potential for ferrocene‐mediated catalysis of the generated hydrogen peroxide was 0.25 V vs. Ag/AgCl, an improvement on sensors reported in the literature. The best assembly in terms of sensitivity and detection limit of 66 µM was found to be one using an external Nafion coating. Interferences were generally small. This sensor was applied to the determination of glucose in sweet wine samples by the standard addition method.
Financial support from Fundação para a Ciência e Tecnologia (FCT), ICEMS (Research Unit 103) and European Project HPRN‐CT‐2002‐00186 is gratefully acknowledged. M.E.G. thanks FCT for a Ph.D. grant (SFRH/BD/14014/2003). Prof. H.‐D. Liess is thanked for the gift of the electrical resistors.
Notes
Financial support from Fundação para a Ciência e Tecnologia (FCT), ICEMS (Research Unit 103) and European Project HPRN‐CT‐2002‐00186 is gratefully acknowledged. M.E.G. thanks FCT for a Ph.D. grant (SFRH/BD/14014/2003). Prof. H.‐D. Liess is thanked for the gift of the electrical resistors.