A Mediatorless Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Immobilized in Tin Oxide Sol‐Gel Film

Abstract

A novel unmediated hydrogen peroxidase biosensor was developed by immobilizing horseradish peroxidase (HRP) into a tin oxide sol‐gel film at a glass carbon electrode surface. Morphology of the sol‐gel film and HRP entrapped in the sol‐gel film were characterized by transmission electron microscopy (TEM) and Fourier‐transform infrared (FTIR) spectroscopy, respectively, showing the uniform porous network structure in the presence of nano‐SnO₂ particles and the retention of the enzymatic bioactivity with its native secondary structure. The direct electrochemistry of HRP incorporated in the film was investigated using cyclic voltammetry. The biosensor exhibited good stability and electrocatalytic activity toward the reduction of hydrogen peroxide. The linear concentration range of the biosensor was from 0.01 mM to 0.25 mM. The apparent Michaelis‐Menten constant K _M ^app was estimated to be 0.166 mM.

Keywords:

• Horseradish peroxidase
• tin oxide sol‐gel film
• biosensor

This work was supported by the Shanghai Rising Star Project of Shanghai Science and Technology Committee (No. 04QMX1443), the Excellent Young Teachers Candidates Program for Higher Education from Shanghai Municipal Education Commission, China.