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Abstract
Urine is a diagnostically important biological fluid. As a complex mixture of different metabolites where numbers of them are natural fluorophores, its composition could be defined graphically by excitation‐emission or synchronous fluorescence spectrum (SFS). Interpretation and practical application of such spectral characterization are significantly complicated due to the concentration variability of solitary urines. The most critical is the evaluation of extremely concentrated (dense) or diluted urines (some physiological/pathological states). For elimination of this undesirable effect on spectral shape we introduced a new method of fluorescence evaluation—concentration matrices. They are created after synchronous spectral scanning (Δλ=30 nm) of a broad concentration scale of individual urines and a mathematical alignment of all scans. The 3‐D contour graph formed by this procedure represents the relation among the fluorescence intensity, excitation wavelength, and urine dilution. The shape of contours enables the researcher to better distinguish the content, including fluorophores and their interactions. The SFS concentration matrices significantly improve fluorescence analysis, fingerprinting, and definition of urine.