Screening and identification of microorganisms capable of utilizing phosphate adsorbed by goethite

Abstract

Information on phosphorus (P)-solubilizing microorganisms in variable charge soil is lacking. We screened soil microorganisms that can effectively utilize P adsorbed on variable charge minerals using a series of synthetic media of decreased P availability. Rhizospheric soil (Orthic plintaqualt) from a 30-y old tea plant was diluted and aseptically inoculated to a series of media containing P adsorbed on goethite at 0, 255, 50, 75, and 100% saturation. Microorganisms, which survived in the 25% P sorption saturation medium, were each by colony isolates transferred to another freshly prepared medium of the same type for growth stability test. Microbial species, which could grow and reproduce for more than 15 generations in the 25% P-saturation medium were considered as P-solubilizing microbes (PSMs) and used for identification tests. By this procedure, we screened one PSM population which was identified as the bacterium Moraxella sp by Gram staining, Gamma culture and staining, optical and electron microscopic observation, and enzyme-oxidizing reaction. The PSMs have higher maximal transport rates to P than the ubiquitous soil microorganisms and have an optimal temperature of 37°C and optimal pH of 5.5–7.5 for growth.

Keywords:

• Bacteria
• Identification
• Phosphorus solubilization
• Variable-charge minerals

Acknowledgments

This study was, in part, supported by the Outstanding Young Scientist Fund from The Natural Science Foundation of China with approval grant No. 40025104.