Normal-Phase TLC Separation of Enantiomers of 1.4-Dihydropyridine Derivatives

Abstract

A new TLC-based method was proposed for the separation of enantiomers and mixtures of racemic DHP derivatives differing in the kind of substituent in the phenyl ring. The conditions for the effective determination of the substances involved and the mechanism of their sorption were also studied. For the separation of felodipine, nilvadipine, and isradipine enantiomers, thin-layer chromatography was used, with a chiral stationary phase of the ligand exchange type, and developing phases of a different concentration of methanol (Φ) as an organic modifier. The retention coefficient values k′ were used to make the plots log k′ = f (log Φ) and log k′ = f (Φ). The processes taking place in the chromatographic systems were shown to be described by the Snyder–Soczewiński equation.