Stability Indicating Method for SPf66 Antimalarial Peptide in Solution

Abstract

Stability studies on the SPf66 antimalarial peptide with different pH and temperature conditions were carried out. The degradation mechanism was elucidated by the size‐exclusion chromatography (SEC) technique and the experimental data obtained at 37°C and different pH were fitted to a kinetic degradation model that could explain the loss of its immunogenic capacity. At 5, 25, 37, and 70°C and pH 2, changes were detected in the areas of the different species, although the values obtained could not be fitted to any known degradation kinetics.

Key Words:

• Degradation kinetics
• Dissolution
• Oxidation
• SEC
• SPf66 synthetic peptide
• Stability studies