Synergic and Competitive Inhibition Effect BetweenTwo Kinds of Arylamines in the Formation of Hemoglobin Adduct In Vitro

Abstract

This article presents our work with HPLC-UV to determine hemoglobin adducts produced by adduction reaction among 4-aminobiphenyl (4-ABP) or/and 2-naphthylamine (2-NA) and human hemoglobin in vitro. The results showed that HPLC-UV could efficiently be used to detect aromatic amine-hemoglobin adduct. Two kinds of optimized linear models modified from the traditional dose-response model were screened out. The models implied that synergic effect exists between the two kinds of arylamines adducted with hemoglobin and competitive inhibition effect occurs in the formation of hemoglobin adduct when the two kinds of arylamines are present in the blood synchronously. The results indicate that models for dose-response relationship should take the effects of the other toxicants into consideration in the mixed pollutant system.

Keywords:

• Arylamines
• Hemoglobin adduct
• Model screening
• Synergic effect
• Competitive inhibition effect

Acknowledgments

Financial supports from NSFC (29837180), CAS (KJ 952-J1-520) and 9906 are gratefully acknowledged. Authors would also like to thank the great support for the revision of the paper from Prof. Z. M. Ni.