Kinetics of LCFA Inhibition on Acetoclastic Methanogenesis, Propionate Degradation and β-Oxidation

Abstract

Kinetics of long-chain fatty acids (LCFAs) inhibition on acetoclastic methanogenesis, propionate degradation and β-oxidation were studied with granular sludge under mesophilic batch conditions. Mathematical expressions used for reaction rates were as shown below:

The simulated results revealed that the methane production rates from acetate decreased with an increase in both concentration and the number of double bonds of LCFAs. The concentrations of oleate (C18:1), linoleate (C18:2), palmitate (C16:0), and stearate (C18:0) were 0.54 mM, 0.11 mM 1.62 mM, and 2.58 mM, respectively, at which the methane production rates from acetate dropped 10%, and 3.10 mM, 0.72 mM, 5.71 mM, and 5.37 mM, respectively, at which the rates dropped 50%. The inhibitory effects of LCFAs on propionate degradation showed a similar tendency with acetoclastic methanogenesis; however, were less severe. The concentrations of oleate, linoleate, palmitate, and stearate were 1.02 mM, 0.18 mM, 2.34 mM, and 1.92 mM, respectively, at which the propionate degradation rates dropped 10%, and 4.38 mM, 1.17 mM, 5.88 mM, and 5.18 mM, respectively, at which the rates dropped 50%. The observed maximum β-oxidation rates of oleate, linoleate, palmitate, and stearate were 0.21 mmol (g VSS)⁻¹ d⁻¹, 0.09 mmol (g VSS)⁻¹ d⁻¹, 0.12 mmol (g VSS)⁻¹ d⁻¹, and 0.08 mmol (g VSS)⁻¹ d⁻¹, respectively. The lag-phase times in β-oxidation were also dependent on LCFA concentrations. The concentrations of oleate, linoleate, palmitate, and stearate, at which the lag-phase times became 5 days, were 5.93 mM, 2.24 mM, 4.02 mM, and 2.81 mM, respectively.

Keywords:

• Long-chain fatty acids (LCFAs)
• Inhibition
• Acetoclastic methanogenesis
• β-Oxidation
• Propionate

Acknowledgments

This work was supported by grant No. M1-0203-00-0063 from the National Research Laboratory Program of the Korean Ministry of Science and Technology.