INHIBITORY EFFECT OF TUMOR NECROSIS FACTOR-α SECRETION FROM RAT ASTROCYTES BY CHILBOKEUM

Abstract

Substance P (SP) can stimulate secretion of tumor necrosis factor-α (TNF-α) from astrocytes stimulated with lipopolysaccharide (LPS). In this study, we have examined whether an aqueous extract of Chilbokeum inhibits secretion of TNF-α from primary cultures of rat astrocytes. Chilbokeum (10 μg/ml) significantly inhibited the TNF-α secretion by astrocytes stimulated with LPS and SP. Interleukin-1 (IL-1) has been shown to elevate TNF-α secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore examined whether IL-1 mediated inhibition of TNF-α secretion from primary astrocytes by Chilbokeum. Treatment of Chilbokeum (10 and 100 μg/ml) to astrocytes stimulated with both LPS and SP decreased IL-1 secretion significantly. Moreover, the secretion of TNF-α by LPS and SP in astrocytes was progressively inhibited with increasing amount of IL-1 neutralizing antibody. Our results suggest that Chilbokeum may inhibit TNF-α secretion by inhibiting IL-1 secretion and that Chilbokeum has an antiinflammatory activity in the central nervous system.

Key Words :

• Chilbokeum
• Tumor necrosis factor-α
• Astrocytes
• Lipopolysaccharide
• Substance P
• Interleukin-1