Mechanical Fluid Flow Enhances High-Affinity-IgE-Receptor-Mediated Secretion by Mast Cells Adherent to Fibronectin

Abstract

We demonstrated the effect of interaction between mechanical fluid flow and the extracellular matrix on high-affinity-IgE-receptor (FcεRI)-mediated secretion by mast cells (cell line MC/9). Mechanical fluid flow did not affect secretion by the cells in the absence of exogenou agonists. Mechanical fluid flow did not affect secretion by cells adherent to fibronectin in the absence of exogenous agonists, but it did enhance FcεRI-mediated secretion by cells adherent to fibronectin. These finding indicate that fluid-flow-induced enhancement of secretion by mast cells requires both exogenous agonists and interaction between integrins and the extracellular matrix. The present study is the first to demonstrate that mechanical fluid flow enhances FcεRI-mediated secretion by a mast cell line adherent to fibronectin.

• Adhesion to fibronectin
• Mast cells
• Mechanical fluid flow
• Mediator secretion