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Journal of Liquid Chromatography & Related Technologies Volume 24, 2001 - Issue 1
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Original Articles

SIMPLE ASSAY FOR THE DETERMINATION OF FLUMEQUINE AND OXOLINIC ACID IN FISH MUSCLE AND SKIN BY HPLC

Víctor Hormazábal Division of Food Hygiene, The Norwegian School of Veterinary Science, Oslo, Norway
&
Magne Yndestad Division of Food Hygiene, The Norwegian School of Veterinary Science, Oslo, Norway
Pages 109-115 | Received 18 Jun 2000, Accepted 30 Jul 2000, Published online: 01 Jun 2007
 
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Abstract

A simple method for the simultaneous determination of flumequine and oxolinic acid in fish muscle with attached skin is presented. Samples of mass 3.0 g were extracted with NH3 and acetone, acidified, diluted, and analyzed by high-performance liquid chromatography. The limit of quantification was 30 μg/kg for flumequine and 20 μg/kg for oxolinic acid. The recoveries were 75–76% and 79–82% for flumequine and oxolinic acid, respectively.

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