Abstract
The separation of color pigments of chili powder was performed on various narrow bore HPLC columns (octadedecylsilica, silica, and polybutadiene coated alumina) using gradient elution and, the retention characteristics of the columns were compared. The separation capacities of silica and polybutadiene coated alumina were lower than those of other columns. The highest number of fractions were separated of octadecylsilica columns, however, the efficiency of the column depended considerably on the type of C18 coating, proving the marked influence of coating process on the separation capacity.
ACKNOWLEDGMENT
This work was supported by a Portugues-Hungarian cooperation grant entitled “Development of New Methods and Their Application for the Assessment of the Effect of Environmental Conditions on the Stability of Colour Pigments in Foods”.