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Original Articles

SEPARATION OF COLOR PIGMENTS OF CHILI POWDER ON NARROW-BORE HPLC COLUMNS. A COMPARISON

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Pages 425-433 | Received 30 May 2000, Accepted 28 Jun 2000, Published online: 01 Jun 2007
 

Abstract

The separation of color pigments of chili powder was performed on various narrow bore HPLC columns (octadedecylsilica, silica, and polybutadiene coated alumina) using gradient elution and, the retention characteristics of the columns were compared. The separation capacities of silica and polybutadiene coated alumina were lower than those of other columns. The highest number of fractions were separated of octadecylsilica columns, however, the efficiency of the column depended considerably on the type of C18 coating, proving the marked influence of coating process on the separation capacity.

ACKNOWLEDGMENT

This work was supported by a Portugues-Hungarian cooperation grant entitled “Development of New Methods and Their Application for the Assessment of the Effect of Environmental Conditions on the Stability of Colour Pigments in Foods”.

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