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Journal of Liquid Chromatography & Related Technologies Volume 24, 2001 - Issue 2
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Original Articles

QUANTITATIVE DETERMINATION OF COMMERCIAL OXIDATION HAIR DYES BY REVERSED-PHASE HPLC

B. H. Shao Department of Chemistry, Zhejiang University, Hangzhou, 310027, People's Republic of China
,
X. Z. Xu Department of Chemistry, Zhejiang University, Hangzhou, 310027, People's Republic of China
,
J. W. Yan Department of Chemistry, Zhejiang University, Hangzhou, 310027, People's Republic of China
&
X. Y. Fu Department of Chemistry, Zhejiang University, Hangzhou, 310027, People's Republic of China
Pages 241-249 | Received 10 Jun 2000, Accepted 15 Jul 2000, Published online: 06 Feb 2007
 
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Abstract

A simple and reliable RP-HPLC method for the separation and quantitative determination of p-phenylenediamine, p-aminophenol, m-aminophenol, resorcinol, and 2,6-diaminopyridine in commercial oxidation hair dyes was developed. The separation was carried out on a μ-Bondapak C18 column with methanol-aqueous solution containing 0.1% triethylamine and 0.02 mol l−1 NH4Ac (pH = 5.20) (10:90, vol./vol.) as the mobile phase. Without a complex extraction procedure, the method is sufficiently rapid and accurate for routine analyses. Two kinds of commercial hair dye samples were determined successfully.

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