Abstract
Preparative scale purification of PEG-protein conjugates by centrifugal precipitation chromatography is discussed. By utilizing the transport of ammonium sulfate across a membrane and the centrifugal force, an ammonium sulfate gradient is formed and used for differential precipitation of PEG-protein conjugates. Native proteins can be separated from PEG-protein conjugates by using this technique.
ACKNOWLEDGMENT
One of the authors, Terasut Sookkumnerd, is greatly indebted to Suranaree University of Technology, Thailand and Ministry of University Affairs, Royal Thai Government for providing financial support under the Faculty Development Program during his study at Lehigh University.