APPLICATION OF CENTRIFUGAL PARTITION CHROMATOGRAPHY IN A GENERAL SEPARATION AND DEREPLICATION PROCEDURE FOR PLANT EXTRACTS

Abstract

Centrifugal Partition Chromatography (CPC) was used in a general separation and dereplication procedure in search for new biologically active compounds from crude plant extracts. In this procedure, the alcoholic extract was prefractionated by CPC with the solvent system heptane/ethyl acetate/methanol/water 6:1:6:1 (v/v/v/v) followed by bioactivity screening of the fractions. The active fractions were analyzed for the known active components for dereplication. If the activity was found in the most polar fraction, which tended to contain a large group of compounds, the fraction was separated again by CPC (solvent system ethyl acetate/methanol/water 43:22:35, v/v/v). This two-step procedure was found to be efficient for five extracts tested for both chemical and bioactivity profiles.

ACKNOWLEDGMENTS

Professor A. P. IJzerman, LACDR, The Netherlands is acknowledged for providing the facilities for the radio-ligand binding assays. The authors also would like to thank Mr. A. Ouanaroon, Faculty of Pharmaceutical Sciences, Naresuan University, Thailand for providing the alcoholic extract of S. siamea, W.F. Leenen & Zn., Sassenheim, The Netherlands for the Narcissus bulbs, Mr. S. Wahyuono, Gadjah Mada University, for the plant materials from Indonesia, Mr L.C. Verhagen from Heineken Technical Services, The Netherlands for the phenolic compounds. The Royal Thai Government is gratefully acknowledged for providing the Ph.D. scholarship awarded to K. Ingkaninan.