374
Views
10
CrossRef citations to date
0
Altmetric
Original Articles

ESTIMATION AND ISOLATION OF AZADIRACHTIN-A FROM NEEM [AZADIRACHTA INDICA A. JUSS] SEED KERNELS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

, , , , &
Pages 2225-2235 | Received 20 Aug 1999, Accepted 08 Feb 2000, Published online: 06 Feb 2007
 

Abstract

An improved reverse phase high performance liquid chromatography (HPLC) procedure has been described to estimate azadirachtin-A content in crude methanolic extracts of neem seed kernels using valley-to-valley integration techniques. The present paper highlights a detailed study of extraction, estimation, and purification of azadirachtin-A present in neem seed kernels. We also present, herein, our results of Soxhlet extraction of Aza-A from the defatted neem seed cake as a function of time, which is found to be more efficient than the conventional extraction procedure.

ACKNOWLEDGMENTS

Authors thank Sun Pharmaceuticals Advanced Research Center (SPARC), Vadodara, for providing PMR spectrum of sample of Azadirachtin and Drs. K. A. Patel and A. K. Varshney for their help. PRU thanks GSFC Science Foundation, GSFC, Vadodara (India) for award of a research fellowship.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 583.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.