ESTIMATION AND ISOLATION OF AZADIRACHTIN-A FROM NEEM [AZADIRACHTA INDICA A. JUSS] SEED KERNELS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

An improved reverse phase high performance liquid chromatography (HPLC) procedure has been described to estimate azadirachtin-A content in crude methanolic extracts of neem seed kernels using valley-to-valley integration techniques. The present paper highlights a detailed study of extraction, estimation, and purification of azadirachtin-A present in neem seed kernels. We also present, herein, our results of Soxhlet extraction of Aza-A from the defatted neem seed cake as a function of time, which is found to be more efficient than the conventional extraction procedure.

ACKNOWLEDGMENTS

Authors thank Sun Pharmaceuticals Advanced Research Center (SPARC), Vadodara, for providing PMR spectrum of sample of Azadirachtin and Drs. K. A. Patel and A. K. Varshney for their help. PRU thanks GSFC Science Foundation, GSFC, Vadodara (India) for award of a research fellowship.