Advanced search
Publication Cover
Journal of Liquid Chromatography & Related Technologies Volume 23, 2000 - Issue 14
Submit an article Journal homepage
31
Views
6
CrossRef citations to date
0
Altmetric
Original Articles

A SIMPLE METHOD FOR DETERMINATION OF NIMESULIDE IN RAT BLOOD SAMPLES BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

M. C. Carrasco-Portugal Instituto Nacional de Pediatría, Secretaría de Salud, Apartado Postal 22026, México, D. F., 14000, Mexico
,
V. Granados-Soto Departamento de Farmacología y Toxicología, Centro de Investigación y de Estudios Avanzados, del Instituto Politécnico Nacional, Apartado Postal 22026, México, D. F., 14000, Mexico
,
G. A. Camacho-Vieyra Instituto Nacional de Pediatría, Secretaría de Salud, Apartado Postal 22026, México, D. F., 14000, Mexico
,
J. Pérez-Urizar Departamento de Farmacología y Toxicología, Centro de Investigación y de Estudios Avanzados, del Instituto Politécnico Nacional, Apartado Postal 22026, México, D. F., 14000, Mexico
&
F. J. Flores-Murrieta Departamento de Farmacología y Toxicología, Centro de Investigación y de Estudios Avanzados, del Instituto Politécnico Nacional, Apartado Postal 22026, México, D. F., 14000, Mexico
Pages 2237-2244 | Received 28 Jun 1999, Accepted 20 Jul 1999, Published online: 06 Feb 2007
 
Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days

Abstract

A rapid and simple method for determination of nimesulide in blood samples, by high-performance liquid chromatography, was developed. This method includes a single extraction of acidified blood (1N hydrochloric acid) with diethyl ether. Blood extracts were analyzed on a reversed-phase column eluted with a mixture of methanol-0.05 M potassium dihydrogen phosphate solution (pH 5) and detected by absorbance at 290 nm.

Retention times for the internal standard and nimesulide were 4.2 and 8.2 min, respectively. The method was linear in the range of 0.2 to 5 μg/mL and the detection limit of the method was 40 ng/mL. This method is suitable for determination of nimesulide after oral administration, and could be used for pharmacokinetic studies of the drug in the rat using small volume samples.

ACKNOWLEDGMENTS

Miriam C. Carrasco-Portugal and José Pérez-Urizar are CONACYT fellows.

Log in via your institution

Access through your institution

Log in to Taylor & Francis Online

Restore content access

Restore content access for purchases made as guest
Purchase options * Save for later

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 583.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.